Gas chromatographic analysis of fatty acids from dialyzed lipoproteins*
نویسنده
چکیده
Dialysis of three major lipoprotein fractions from human serum, Sr20-1W, SP20, and -SI.SOO16, against low concentrations of cupric ion is associated with accelerated chemical alteration of polyunsaturated fatty acids and cholesterol. Leas extensive changes in these acids were observed during dialysis against ion-free water. Reaction products of these chemical alterations were irreversibly retained on succinic aciddiethylene glycol polyester gas chromatographic columns. Present evidence suggests that these reaction products are relatively polar compounds, probably in the chemical class of lipoperoxides.
منابع مشابه
A chromatographic method for detection of palm oil in butter
Replacement with the cheaper animal fats or vegetable oils is conventional in milk through direct incorporation or homogenization of skimmed milk with less expensive foreign fats. The main objective of the present study was to evaluate the capability of a simple, fast, and reliable method for the detection of added palm oil in butter. The butter samples were mixed with palm oil (0.5-50 wt%) fol...
متن کاملDevelopment of gas chromatography analysis of fatty acids in marine organisms.
The gas chromatographic analysis of fatty acids has attracted considerable interest. In this analysis, the common derivatives of fatty acids, such as fatty acid methyl esters, can be detected using a flame ionization detector and the mass spectra can indicate the true structure of fatty acids. This paper reviews gas chromatographic methods for obtaining fatty acids from marine organisms. The st...
متن کاملMonitoring of lipoprotein oxidation by gas chromatographic analysis of hydroxy fatty acids.
We describe a method developed for the quantitative analysis of hydroxy fatty acids derived from fatty acid monohydroperoxides formed during lipoprotein oxidation. The procedure starts with catalytic hydrogenation of the lipid extract, whereby hydroperoxyl groups are converted to hydroxyl groups and double bonds are eliminated, and the risk for lipid oxidation during the rest of the procedure i...
متن کاملStudies on Chemical Composition and Nutritive Evaluation of Wild Edible Mushrooms
Agaricus bisporus and Agaricus bitorquis are among most favorite mushrooms of the world. Mineral contents, proximate composition and fatty acid profile of these mushrooms were determined for evaluation of their dietary value. Na, K and Li contents were high, while Cr, Cu, Pb, Co, Zn, Mn and Ni concentrations were low in both Agaricus bisporus and Agaricus bitorquis. Proximate analysis showe...
متن کاملSeasonal variations in the liver and muscle fatty acid composition of Barbus luteus from Karkhe river (khouzestan , Iran)
from Karkhe river (khouzestan , Iran) were determined. 84 sample were collected in the middle month of each season and Total fatty acid compositions were investigated by a gas chromatographic method. . The most abundant fatty acids in the (SFAs) of the liver and muscle were at the highest in the autumn and spring, respectively. The total monounsaturated fatty acids (MUFAs) were at the highest ...
متن کامل